Figure 4: Transduction of the EGFRvIII+ over-expressing ovarian cancer cells with the DNA constructs for the human recombinant DNase1, DNase1L3, DNase2, DFFB (hrDNases) resulted in their surfaces’ topographies disfigured by multiple blebs (C,D). These could be compared for the surface blebs, which occurred as the results of ROS-induced apoptosis in the cultured OVCAR cells (A,B). Pores in the membranes’ blebs, which are only seen at seen at high magnifications, are the routes of entry for labels targeting their content. Presence of these pores in the blebs’ membranes explains observations that the blebs’ contents are labeled long before the cells’ interiors. HFW A,C: 20 μm; B,D: 7.2 μm.