Figure 4: Signal transduction pathways regulation of OPG expression. (A) Effect of PI3K-AKT pathway inhibitor in ovarian HEY cells OPG secretion. Cells were treated with PI3K inhibitors (10mM of LY294002 or PI103), AKT inhibtor (10mM of GDC0941 or MK2206), or 10mM GSK3 inhibitor (GSKi) SB26763 for 24h before media collection; *, p < 0.05 vs pcDNA control). Effect of MAPK pathway inhibitor in OPG secretion in ovarian HEY (B) or MCF7 (C) cells. Cells were treated with MEK inhibitors (10mM of PD98059 or AZD6244). *, p < 0.05 vs no drug control.