Figure 6: EGFR inhibitor promotes cell death induction by TRAIL. (A) Expression of EGFR and ERBB2 in HEY and MCF7 cells was detected by qPCR and western blotting analysis. EGFR level in MCF7 cells was set to 1 for comparison; *, p < 0.05 HEY vs MCF7 cells. EGFR inhibitors increased cell sensitivity to TRAIL-induced cell death in ovarian HEY (B) and breast MCF7 (C) cells. Cells were pre-treated with either 1mM of Lapatinib or Neratinib for 8h before addition of TRAIL in the present or absent of OPG (100ng/ml). a, p < 0.05 vs no drug control; b, p < 0.05 vs TRAIL only without EGFR inhibitor; c, p < 0.05 vs TRAIL alone in the present of Lapatinib or Neratinib.