Figure 7: A) Uptake and cytotoxicity studies of NPs on A549 cells after 4 hours incubation. Cells were stained with Alexa Fluor 594 (blue fluorescence – nucleus) and with Hoechst 33342 (red fluorescence – plasmatic membrane). Images representing two different assays performed in duplicate. A1) Chitosan/ PCL nanoparticles, produced with Chitosan covalently linked to FITC. A2) PCL nanoparticles adsorbed with BSA covalently linked to FITC. B) Flow cytometry analysis of the uptake (FITC) and cytotoxicity (PI) on A549 cells after 4 hours incubation. 1) Simple Chitosan/PCL nanoparticles. 2) Chitosan/PCL nanoparticles adsorbed with HSA. 3) Chitosan/PCL nanoparticles complexed with pDNA. Variations on the concentration of nanoparticles ranged from 1000 to 100 μg/mL. (Error bars represent standard deviation of the mean, n=3).\