T cells isolated from murine splenocytes using the T Cell isolation kit were co-stimulated with PMA (5nM) and ionomycin (1 μg/ml). After 24 hrs, miRNA mimic targeted at cyclophilin B was transfected into the activated primary T cells using HVJ-E vectors. Real-time PCR evaluation (A) and western blot analysis (B) were performed at 48 hrs after the transfection
Figure 2: Delivery of miRNA into activated T cells using HVJ-E vectors.