B cells isolated from murine splenocytes using the B cell isolation kit were costimulated with CD40 antibody/IL-4 or CD40 antibody/LPS. After 24hrs, miRNA mimic targeted at cyclophilin B was transfected into the activated primary B cells using HVJ-E vectors. Western blot analysis in the B cells activated by CD40 antibody/IL-4 (A) or CD40 antibody/LPS (B) was performed at 48hrs after the transfection
Figure 3: Delivery of miRNA into activated B cells using HVJ-E vectors.