AO Number AO coordinates Sequence (5’-3) 1 Hi47A(-05+20) caccuggcucca ccc uugaccugu g 2 Hi47A(+22+46) auccucuuccaugauucaauuauc u 3 Hi47D(+11-14) cuugugaaacuuacugauuau cag g 4 Hi47A(+05+29) aauuaucugcaccuggcucca ccc u 5 Hi47A(-01+24) ucugcaccuggcuccacccuugac c 6 Hi47A(-10+15) ggcuccacccuugaccugugggga u 7 Hi47A(-15+10) cacccuugaccuguggggauuacc a 8 Hi47A(-20+5) uugaccuguggggauuaccauaau u 9 Hi47A(-25-01) cuguggggauuaccauaauucaag g 10 Hi47A(+47+72) ugauuaucaggagaa cag uauggggg 11 Hi47A (10+20) caccuggcucca ccc uugaccuguggggau 12 Hi47A(-5+25) aucugcaccuggcuccacccuugaccugug 13 Hi47A(-01+29) aauuaucugcaccuggcucca ccc uugacc 14 Hi47A(-02+20) caccuggcucca ccc uugacc u 15 Hi47A(-05+17) cuggcucca ccc uugaccugu g 16 Hi47A(+1+22) ugcaccuggcuccacccuuga c 17 Hi47A(+5+26) uaucugcaccuggcucca ccc u

Table 1: AO sequences used in this study and annealing coordinates relative to the pseudo-exon acceptor (A) and donor (D) splice sites; The AO nomenclature is based on that described previously [24] with the Hi47 referring to Human dystrophin intron 47 sequence and the numbers indicating intronic sequences either side of the pseudo-exon.