Figure 2A: WCP hybridization analysis: Using chromosome 21 probe showed two independent green signals on both the normal chromosome(s) 21 (white arrows) and no hybridization on marker chromosome (red arrow) B: Using chromosome 22 probe showed two independent green signals on normal chromosome(s) 22 (white arrows) and also an intense green signal on marker chromosome (red arrow) thus revealed the origin of marker chromosome with sequences of chromosome 22 C: LSI hybridization analysis: Acro-p arm probe showed interestingly two orange signals of Acro-p region on marker chromosome on either side of its centromere (indicated with red arrow) D: NOR staining showed the dark silver staining on marker chromosome on either side of the centromere (indicated with red arrow). Probe hybridization with Acro-P and NOR staining showed satellite regions on all other acrocentric chromosomes E: LSI hybridization analysis: LSI Di-George probe showed two control signals (Green) and two LSI Di-George signals (Orange) on normal chromosome(s) 22 (white arrows) and it shows NO signal neither the control signals nor Di-George signals on marker chromosome (red arrow)