Figure 2: Morphology of skeletal muscle and liver following common freezing techniques.Liver and muscle (tibialis anterior or quadriceps) tissue from C57BL/6 mice was frozen as described and sectioned at 10 μm. (AC) Hematoxylin and eosin staining of liver sections embedded in OCT and frozen with dry ice alone (A), a slurry of dry ice and isopropanol (B), or LqN2- cooled isopentane (C). (D-F) Hematoxylin and eosin stains of skeletal muscle frozen with in OCT dry ice alone (D), a slurry of dry ice and isopropanol (E), or mounted on a wooden dowel and snap-frozen in LqN2-cooled isopentane (F).