Figure 2: Mouse eye (Iris)-derived NG2-expressing progenitors (eye (Iris)- NG2+cells) differentiate into functional Opsin+core neuron in response to injured eye homogenates. Mouse eye Iris from two C57Bl/6J mice (> 8 wks) was used to obtain NG2+cells. The rest of procedures to isolate NG2+cells were performed as described above. NG2+cells were cultured in the presence of homogenates derived from eye injured mice. Representative images show that the isolated NG2+cells differentiated into functional Opsin+core neuron when cultured in the presence of homogenates derived from Iris mice (eye insults).