Figure 3: Improved method of large scale production of HDAd. Overview of rescue (passage 0 or P0), amplification (P1 to P3) and large-scale (P4) production of HDAd. P0 involves transfecting producer cells with pHDAd followed by infection with the HV. Each subsequent serial passage involves coinfecting producer cells with the HDAd and HV. The entire process can take as little as two weeks with total yields of >1013 viral paricles (vp) per 3 liter culture and specific yields of >10,000 vp/cell.