Gene                                 Primer Sequences(5’-3’) length Nrf2 Forward 5’-CACATTCCCAAACAAGATGC-3’ 374 Reverse 5’-TCTTTTTCCAGCGAGGAGAT-3’ Cu-ZnSOD Forward 5’-ATGGCGATGAAAGCGGTGTG-3’ 465 Reverse 5’-TTACTGCGCAATCCCAATCACTC-3’ MnSOD Forward 5’-ATGTTGTGTCGGGCGGCG-3’ 669 Reverse 5’-TCACTTCTTGCAAGCTGTGTATCTTTCA-3’ GSTA1 Forward 5’-GACTGCTTTCTTCAGGGTTCAAG3’ 111 Reverse 5’-TCTGTGTAATTCATGGCTGATTCC-3’ GSTM1 Forward 5’-CGACGCTCCCGACTATGACA-3’ 182 Reverse 5’CACGAATCCGCTCCTCCTCT-3’ GAPDH Forward 5’-ACCACAGTCCATGCCATCAC-3’ 452 Reverse 5’-TCCACCACCCTGTTGCTGTA-3’

Table1: The sequences of primers and amplification length of amplified genes.