| Parameter
|
SD of peak area |
% RSD |
| Mobile phase composition |
0.90 |
1.01 |
| Amount of mobile phase |
1.60 |
1.89 |
| Temperature |
0.60 |
0.90 |
| Relative humidity |
1.26 |
1.81 |
| Plate pretreatment |
0.79 |
0.08 |
| Time from spotting to chromatography |
1.10 |
0.91 |
| Time from chromatography to scanning |
0.08 |
0.05 |
an = 6, Average of three concentrations 200, 600, 1000 ng/spot |
