S.no

Batch code

CH %
w/v

Cross linking agent

LMT
%

PEG
(ml)

Particle size (nm) Mean±SD*

PDI
Mean±SD*

ZP (mV) Mean±SD*

ZP (mV)
in 0.1 mol/l KCl

% Swelling Mean±SD*

%EE
Mean±SD*

Release kinetics
Higuchi

R2

n-value

1.

A1

0.8

10 ml TPP

-

-

111±16.2

0.231±0.049

38±1.2

-2.76±0.21

428±12.6

-

-

-

2.

A2

0.8

10 ml HMP

-

-

136±11.4

0.116±0.039

32±1.7

-2.19±0.16

465±12.7

-

-

-

3.

A3

0.8

10 ml TPP

1

-

478±13.1

0.172±0.032

56±1.1

-3.09±0.1

437±13.4

58±0.88

0.9924

0.49

4.

A4

0.8

10 ml HMP

1

-

510±14.2

0.318±0.027

50.8±2.3

-1.80±0.07

482±14.2

64±0.60

0.9976

0.52

5.

A5

0.8

15 ml TPP

1

-

249±15.1

0.223±0.014

43±1.2

-2.15±0.14

369±11.4

81±0.67

0.9984

0.59

6.

A6

0.8

15 ml HMP

1

-

458±13.9

0.199±0.059

46±1.5

-1.05±0.19

375±10.4

87±0.85

0.9939

0.57

7.

A7

0.8

10 ml TPP

-

5

265±12.8

0.517±0.037

36±1.0

-1.86±0.09

350±13.2

-

-

-

8.

A8

0.8

10 ml HMP

-

5

388±17.6

0.263±0.022

29±1.1

-1.32±0.05

395±14.4

-

-

-

9.

A9

0.8

10 ml TPP

1

5

717±19.3

0.121±0.036

46±0.96

-3.47±0.17

455±15.3

76±0.90

0.9899

0.50

10.

A10

0.8

10 ml HMP

1

5

942±11.7

0.276±0.031

42±1.4

-2.76±0.2

478±15.2

79±0.48

0.9913

0.53

11.

A11

0.8

15 ml TPP

1

5

571±17.8

0.224±0.026

37±1.7

-3.85±0.06

347±14.8

85±0.82

0.9932

0.55

12.

A12

0.8

15 ml HMP

1

5

648±16.3

0.142±0.046

35±2.2

-2.94±0.07

392±13.7

96±0.51

0.9963

0.58

PEG: Polyethylene glycol 6000, TPP: Sodium tripolyphosphate, HMP: Sodium hexametaphosphate, LMT: Lomustine, CH: Chitosan, PDI: Polydispersity index; SD:
Standard deviation for three determinations.
*n = 3. The experiments were repeated twice.
Table 1: Formulation composition and characterization of DNPs and PEG coated DNPs.