Figure 7: Bright field (A, D) and fluorescent (B, C: C4-2B green, HS-5 - orange) images of co-culture of prostate cancer C4-2B and stromal HS-5 cells. (A, B): before exposure to the pair of laser pulses (532 nm and 787 nm); (C, D): 60 s after exposure to the laser pulses that were scanning along the area shown with a dashed line. Fading of green fluorescence was due to the leakage of green calcein dye out of the damaged C4-2B cell through its disrupted membrane (shown with yellow arrow). Yellow arrows show a single cancer cell that has been selectively damage with the rainbow mechanism of PNB generation.