Formula

Preparation Technique

 

Protein loading protocol

Polymer solution
(%w/v)

Crosslinker
(% w/v)

N1

Precipitation

Adsorption

CS + 1% Tween 80

20 %  Na2SO4

N2

Adsorption

CS + 1% Tween 80

20%  Na2SO4 +
1 ml 25% GA

N3

Entrapment

CS + 1% Tween 80 + (0.02 %) HSA

10 %  Na2SO4

N4

Adsorption

CS + 1% Tween 80

10 % Na2SO4

N5

Adsorption

CS  + 1% Tween 80

10 % TPP

N6

Entrapment

CS + 1% Tween 80 + (0.02%) HSA

10 % TPP

N7

Ionic-gelation

plain

CS

0.1 % TPP
(3:1 CS/TPP)

N8

Entrapment

CS + (0.02%) HSA

0.1 % TPP
(3:1 CS/TPP)

N9

Entrapment

CS + (0.02%) HSA

0.1% TPP
(5:1 CS/TPP)

N10

Entrapment

CS + (0.02%) HSA

0.1% TPP
(7:1 CS/TPP)

Table 1: Composition of Chitosan Nanoparticle Formulations Prepared by Precipitation and Ionic-Gelation Techniques.