Formula Preparation Technique   Protein loading protocol Polymer solution (%w/v) Crosslinker (% w/v) N1 Precipitation Adsorption CS + 1% Tween 80 20 %  Na2SO4 N2 „ Adsorption CS + 1% Tween 80 20%  Na2SO4 + 1 ml 25% GA N3 „ Entrapment CS + 1% Tween 80 + (0.02 %) HSA 10 %  Na2SO4 N4 „ Adsorption CS + 1% Tween 80 10 % Na2SO4 N5 „ Adsorption CS  + 1% Tween 80 10 % TPP N6 „ Entrapment CS + 1% Tween 80 + (0.02%) HSA 10 % TPP N7 Ionic-gelation plain CS 0.1 % TPP (3:1 CS/TPP) N8 „ Entrapment CS + (0.02%) HSA 0.1 % TPP (3:1 CS/TPP) N9 „ Entrapment CS + (0.02%) HSA 0.1% TPP (5:1 CS/TPP) N10 „ Entrapment CS + (0.02%) HSA 0.1% TPP (7:1 CS/TPP)

Table 1: Composition of Chitosan Nanoparticle Formulations Prepared by Precipitation and Ionic-Gelation Techniques.