Figure 2: Characterization of SPARC targeted biomaterial. A. Schematic of the targeted and base nanoparticles. B. TEM micrographs of aminoSPARK nanoparticles. C. AFM height image of aminoSPARK nanoparticles on gold on mica. D. Specificity of the nanoparticles. Increasing log concentrations of SPARC (Biotin) NP were incubated with either C42b SPARC positive cells or DU145 SPARC low or negative prostate cancer cells. After incubation with streptavidin-HRP and addition of TMB, absorbance was read on a plate reader.