Figure 4: Detection of Bone tumors with SPARC NP. Animals were injected intratibially with either C42b or DU145 cells. A. CT (left) and FMT (right) imaging of SPARC NP injected animals. Arrow points to the C42b derived tumor. B. Quantitation of SPARC NP accumulation in either C42b or DU145 derived tumors in the bone vs. the control non-tumored leg of the same mouse. C. Correlative histology from legs with C42b tumors or control non-tumored legs harvested and sectioned after the completion of the imaging experiments. Left, H&E, middle anti-SPARC antibody, and Right fluorescence from SPARC NP. D. Correlative histology from legs with DU145 intratibial tumors.