Figure 8: Real-time visualization of cellular responses to PD153035 in single A431 living cells. (A), (B), and (C) The bright-field cell image of A431 cells before and after 15 and 50 minutes treatment with 1 μΜ PD153035. (D) and (E) The simultaneous QD-EGF fluorescence image after 15 and 36 minutes treatment with 10 nM QD-EGF and 1 μΜ PD153035. (F) The corresponding bright-field cell image of (E). (G) and (H) The simultaneous QD-EGF fluorescence image after 15 and 50 minutes treatment with drug-free medium. (F) The corresponding bright-field cell image of (E). Inset of (D), (E), (G), and (H) represented the fluorescent intensity profile of the cross section of the objects marked by a dashed line.