Figure 4: (a) Cellular uptake of BODIPY 493/503 dye -loaded core-shell nanoparticles by PC3 and PC3-PSMA human prostate cancer cell lines following 5 h treatment. Untreated PC3 and PC3-PSMA cells were used as controls. In all cases, cellular nuclei were stained using Hoechst 33258. The top row shows overlays of green (BODIPY) and blue (Hoechst) fluorescence images. The bottom row shows overlays of fluorescence images and their corresponding differential interference contrast (DIC) images.