Figure 1: Construction of DR-FM2G plasmid DNA. A gene fragment corresponding to the regions containing residues 200-298 of RSV F, residues 78-98 of RSV M2 and residues 145-205 of RSV G was amplified by PCR using the pET-FM2G plasmid. The resulting fragment that included EcoRI and BamHI restriction sites was cloned into the phCMV1 vector resulting in the DR-FM2G plasmid vector.