MATERIAL

METHOD

CONCENTRATON

RESULTS

AUTHOR

 

 

 

 

 

Polyamidoamine-MWCNT-plasmid DNA

Hela cells

50 µg/ml

Decreased cytotoxicity
by 38%

[153]

MWCNT-COOH;MWCNT-NH2

Human astrocyte D384
And lung A549 cells

   1 µg/ml

High solubility and dispersibility,
Reduced toxicity

[154]

MWCNT-COOH,
MWCNT- PVA
MWCNT-Apatite
(PVA: Poly Vinyl alcohol)

MG-63 human osteoblastic cells

 

MWCNT-Apatite shows higher cell viability than MWCNT-COOH and MWCNT-PVA
 

[155]

pEGFPplasmid DNA-SWCNT

Mouse B-cells cortical neurons

0.1pM/10mL

 Only 10% non-viable cells

[156]

Glycopolymer-SWCNT-plasmid
(pEGFP)

HeLa cells

0.1 mg/ml

No toxicity

[157]

 

 

 

 

 

(SWCNT-NH3+;MWCNT- NH3+)lysine
Functionalized with plasmid DNA

A549 cells

150 µg/ml

Good gene delivery vector

[158]

 

 

 

 

 

Plasmid DNA-SWCNT and
Plasmid DNA-MWCNT

HeLa cell line

10mg/ml

Exhibit low toxicity, improved gene expression up to 10 folds.

[22]

FITC-SWCT and
biotin-SA-SWCNT

HL60,3T3,Chinese hamster ovary cell

0.05mg/ml

Both the f-CNTs were internalized inside the cells without toxicity by endocytosis.

[63]

f-CNT prepared by 1,3
cycloaddition and oxidation/
amidation

B&T lymphocytes and macropghages

1-10µg/ml

f-CNTs were uptaken by cells,
highly soluble and preserved the activity of immunoregulatory cells

[159]

SWCNT-phenyl-SO3H,
SWCNT-phenyl- SO3Na,
SWCNT-phenyl-COOH,
SWCNT in pluronic  F108

Cultured human
Dermal fibroblast

3µg/ml-30µg/ml

Reduced in toxicity observed with
Increased of degree of functionalization. f-CNT were less toxic  than surfactant stabilized SWCNT.

[160]

 

 

 

 

 

Pluronic F127(PF127) surfactant
coated MWCT

Mouse cerebral cortex,
Cultured human blastoma cells

0.01%,
5-10mµg/ml

No degeneration of neurons, no
Induction of apoptosis,
Presence of MWCNT reduced toxicity of PF127

[52, 161, 162]
 

MWCNT- NH2

Fibroblast L929 cell line

1-50 µg/ml

Non-toxic but reduced cell growth observed

[163]

 

 

 

 

 

NH2-MWCNT-DNA

HUVEC cell & A375(human melanoma cell line)

60µg/ml

No cell toxicity

[164]

SWCNT-AHA
(AHA: 6-aminohexanoic acid)

HEK cells(human epidermal keratinocytes)

0.00000005-0.05mg/ml

Maintains cell viability

[165]

PEI-MWCNT-DNA

HEpG2 and COS7 cells

1mg/ml

Low toxicity, improved gene expression up to 10 folds.

[166]

Non covalently functionalized with PEG
(PEG-SWCNT)
Oxidized, covalently functionalized PEG
  (PEG- O- SWCNT)
(PEG: Poly ethylene glycol)

mice

100µl

PEG-SWCNT less toxic than PEG O SWCNT

[167]

 

 

 

 

 

MWCNT-COOH,
MWCNT-Amide and
MWCNT- Benezimidazole

Fibroblast cells

 

Functionalized CNTs show more than 50 %  cell viability

[168]

 

 

 

 

 

Table 1: CNT functionalized with different groups and bio-molecules leading to the reduction of cell toxicity.