Figure 4: Fullerene derivatives inhibit secretagogue-induced elevations in intracellular calcium levels. Mast cells were incubated overnight with FD, washed and FURA 2 added to MC and incubated for 20 minutes at 37oC in the dark. After washing cells were activated with optimal concentrations of the indicated secretagogue and intensity of fluorescence was read as a ratio 340 nm and 380 nm wavelengths. Figures show duplicate samples for each condition and are representative of three separate MC cultures.