Figure 5: Inhibition of mediator release in compound 48/80, poly-L-lysine, and somatostatin challenged MC involves the down-regulation of MAPK and LAT phosphorylation. MC were incubated with or without the FD overnight, washed and activated with the indicated secretagogue. Western blotting was performed as described previously in Methods with the indicated Abs and Odyssey IR800-anti-rabbit secondary antibodies. In both cases, the total protein content in lysates was measured with the house keeping gene β-Actin and Odyssey IR680-anti-mouse secondary antibody.