Gene Expression (NRCWE 002) Day 1 Day 2 Day 3
C3                -75% 0 0
IL6 0 0 0
IL10 +200% +200% 0
CXCL2 0 0 0
TNF-α 0 0 0
Fas Ligand +75% 0 0
ICAM-1 +75% 0 0
Albumin 0 0 0
Table 5: mRNA expression of inflammatory cytokines and receptors, FasL and albumin in C57/BL6 mice liver. The animals were exposed to a single dose of 100 μl of 128 μg/ml of the TiO2 NM (NRCWE 002) by injection via the lateral tail vein. The animals were dissected 24 hr, 48 hr and 72 hr after the initial injection and the mRNA expression was analysed by real time PCR, with B2m utilised as an endogenous control.