Figure 4: Size-exclusion chromatographic patterns of GM1/Ptx 20/1 molar ratio micelles incubated with HSA (HSA/GM1 1:1 w/w) at: pH 7 (--------); 5 (— ) and 3 (……..) for 24 h. Then samples of pH 3 and 5 were returned to pH 7 after 24 h. Incubation temperature was 37ºC for all samples.