| |
Soluble Ptx (%) |
| pH |
GM1/Ptx |
GM1/HSA/Ptx |
| 3 |
96 |
54 |
| 5 |
94 |
20 |
| 7 |
94 |
76 |
Note: A solution of Ptx (50 mg.mL-1) in DMSO was slowly added with gentle
agitation to solutions of GM1 and GM1/HSA (1:1 w/w ratio) prepared at different
pH conditions: 3, 5 and 7 generating complexes with GM1/Ptx 20/1 molar ratio.
The mixtures were incubated at 4ºC for 24 h and dialyzed for 24 h at the same
temperature to remove all DMSO. Then, Ptx was measured as described in
Materials and Methods |
