Soluble Ptx (%)
pH GM1/Ptx GM1/HSA/Ptx
3 96 54
5 94 20
7 94 76
Note: A solution of Ptx (50 mg.mL-1) in DMSO was slowly added with gentle agitation to solutions of GM1 and GM1/HSA (1:1 w/w ratio) prepared at different pH conditions: 3, 5 and 7 generating complexes with GM1/Ptx 20/1 molar ratio. The mixtures were incubated at 4ºC for 24 h and dialyzed for 24 h at the same temperature to remove all DMSO. Then, Ptx was measured as described in Materials and Methods
Table 1: Interaction of Ptx with GM1 micelles and GM1 micelles pre-incubated with HSA at different pHs (3, 5 and 7).