Figure 4: Inhibition of PKC activity interfered with 45Ca-apatite uptake in Jurkat cells. Jurkat cells were treated with generated 45Ca-apatite particles in presence of either PMA or cytochalasin D or both PMA and cytochalsin D at a final concentration of 10 nM and 1 μM, respectively. At indicated time points medium were removed, and cells were washed 3 times with 10 mM EDTA in ice cold PBS. Staurosporin (St) was added in the incubation reaction at a final concentration of 3 nM. Cells were immediately processed for PKC activation assay. Error bars, SEM (n=4).