Figure 5: PMA and cytochalasin D treatment enhanced Ca+2 secretion from Jurkat cells. Cells were incubated with either 45Ca-particle (A, and C) or ion (no particle) (B) for either 30 min (A, and B) or 1 hr (C) prior to addition of the specified reagents. At indicated time points media were removed, and cells were washed 3 times with 10 mM EDTA in ice cold PBS. Samples were collected and immediately processed for radioactivity measurements. For C, reagents were added after 3 times washing with 10 mM EDTA in PBS and cells were maintained in RPMI medium devoid of 45Ca-particle.