Recombinant proteins Protein name Gene symbol Protein name Gene symbol IF1 infA GlnRS glnS IF2 infB TrpRS trpS IF3 infC TyrRS tyrS Methionyl-tRNA formyltransferase Fmt HisRS hisS EF-Tu TufA ProRS proS EF-Ts Tsf ThrRS thrS EF-G fusA SerRS serS RF1 prfA LysRS lysS RF2 prfB AsnRS asnS RF3 prfC AspS aspS1 RRF Frr AlaRS alaS ArgRS argS GlyRS glyS CysRS cysS PheRS 22 pheS pheT IleRS ileS Creatine kinase   LeuRS leuS Nucleotide diphosphate. Kinase Ndk MetRS metG Myokinase   ValRS valS Inorganic pyrophosphatase Ppa GluRS gltX T7 RNA polymerase   Ribosomal proteins Protein name Protein name Protein name Protein name 30S ribosomal subunit protein S1 50S ribosomal subunit protein L17 50S ribosomal subunit protein L5 30S ribosomal subunit protein S17 30S ribosomal subunit protein S2 50S ribosomal subunit protein L18 50S ribosomal subunit protein L6 30S ribosomal subunit protein S18 30S ribosomal subunit protein S3 50S ribosomal subunit protein L19 50S ribosomal subunit protein L7/L12 30S ribosomal subunit protein S19 30S ribosomal subunit protein S4 50S ribosomal subunit protein L20 50S ribosomal subunit protein L9 30S ribosomal subunit protein S20 30S ribosomal subunit protein S5 50S ribosomal subunit protein L21 50S ribosomal subunit protein L10 30S ribosomal subunit protein S21 30S ribosomal subunit protein S6 50S ribosomal subunit protein L22 50S ribosomal subunit protein L11 30S ribosomal subunit protein S22 30S ribosomal subunit protein S7 50S ribosomal subunit protein L23 50S ribosomal subunit protein L13 50S ribosomal subunit protein L1 30S ribosomal subunit protein S8 50S ribosomal subunit protein L24 50S ribosomal subunit protein L14 50S ribosomal subunit protein L2 30S ribosomal subunit protein S9 50S ribosomal subunit protein L25 50S ribosomal subunit protein L15 50S ribosomal subunit protein L3 30S ribosomal subunit protein S10 50S ribosomal subunit protein L27 50S ribosomal subunit protein L16 50S ribosomal subunit protein L4 30S ribosomal subunit protein S11 50S ribosomal subunit protein L28 50S ribosomal subunit protein L32 30S ribosomal subunit protein S13 30S ribosomal subunit protein S12 50S ribosomal subunit protein L29 50S ribosomal subunit protein L33 30S ribosomal subunit protein S14 50S ribosomal subunit protein L35 50S ribosomal subunit protein L30 50S ribosomal subunit protein L34 30S ribosomal subunit protein S15 50S ribosomal subunit protein L36 50S ribosomal subunit protein L31 30S ribosomal subunit protein S16   Ribosomal RNAs Protein name Protein name Protein name   23S rRNA 5S rRNA 16S rRNA   bulk tRNAs Protein name Protein name Protein name Protein name tRNAalaT tRNAmetT tRNAglyX tRNAglnX tRNAalaU tRNAmetU tRNAglyY tRNAgltT tRNAalaV tRNAmetV tRNAhisR tRNAgltU tRNAalaW tRNAmetW tRNAileT tRNAgltV tRNAalaX tRNAmetY tRNAileU tRNAgltW tRNAargQ tRNAmetZ tRNAileV tRNAglyT tRNAargU tRNApheU tRNAileX tRNAtyrU tRNAargV tRNApheV tRNAileY tRNAtyrV tRNAargW tRNAproK tRNAleuP tRNAvalT tRNAargX tRNAproL tRNAleuQ tRNAvalU tRNAargY tRNAproM tRNAleuT tRNAvalV tRNAargZ tRNAsec tRNAleuU tRNAvalW tRNAasnT tRNAserT tRNAleuV tRNAvalY tRNAasnU tRNAserU tRNAleuW tRNAvalZ tRNAasnV tRNAserV tRNAleuX tRNAglyU tRNAasnW tRNAserW tRNAleuZ tRNAglyV tRNAaspT tRNAserX tRNAlysQ tRNAglyW tRNAaspU tRNAthrT tRNAlysT tRNAglnV tRNAaspV tRNAthrU tRNAlysV tRNAglnW tRNAcysT tRNAthrV tRNAlysW tRNAtrpT tRNAglnU tRNAthrW tRNAlysY tRNAtyrT tRNAlysZ tRNAvalX

Table 1: E. coli IVTT components.