Figure 3: Cytotoxicity of doxorubicin micelles against MCF7, MDA-MB-231, DU145 and PC3 cells following 72 hours of treatment. Cell number was determined using the sulforhodamine B assay. Data are expressed as mean ± SEM (n=3). * P < 0.005 relative to 14.5% and 28.4%, † P< 0.005 relative to 28.4%. Non-linear regression and IC50 values determination was performed using GraphPad Prism. Cells were treated with various concentrations of the free drug and SMA-Dox micelles for 72 h.