Figure 6: Histological sections subjected to immunocytochemistry for the detection of the cell proliferation marker PCNA. Slides were counterstained with Harris hematoxylin. PCNA immunostaining of epithelial cells in buccal pouch tissue of male hamsters in the (A-C) Normal, (D-F) Cancer, (G-I) MNP, (J-L) MNP+AMF1 and (M-O) MNP+AMF7 groups. Red arrow: marking limited to the basement membrane of epithelium. Black arrow: atypical mitosis and dispersed marking throughout the tissue, with increased PCNA expression. Scale bars represent 10 μm. Epi: epithelium. Krt: keratin layer. Mit: mitosis. (P) Densitometric analysis. Data are the mean ± SD. Within graphs, columns with different superscript letters differ significantly (p<0.05).