Label  BSA Blocking buffer Casein Blocking buffer
System NB Fluorescent Intensity (a.u.) NB Fluorescent Intensity (a.u.)
FDA@SDS-IgG-Casein 835 ± 46.6 146.5 ± 45
FDA@SDS-IgG-BSA 1506 ± 414.8 418.3 ± 46.2
(1) The Filter set was 470 nm excitation with 515 Long pass emission; the exposure time was 100 ms with the intensifier profile Math DC 500 and Cath - 50.
(2) NB: Nonspecific Binding (Unit: a.u.).
Table 1: Nonspecific binding in different labels and blocking buffers system(1)(n=4).