Figure 9: Externalization of phosphatidylserine in synthesized Ag NPs treated promastigotes. L. donovani promastigotes were incubated with different concentrations of synthesized Ag NPs (a) control (b) 12.5 μg/mL (c) 25 μg/mL (d) 50 μg/mL for 24 h and analysed by flow cytometry. After indicated incubation time, a significant number of membrane compromised cells were stained positively by annexin V- FITC, PI (upper-right quadrant) and only PI positive (upper-left quardrant) (e) Graph showing percentage population of live, apoptotic and necrotic state of parasites treated with different concentrations of synthesized Ag NPs.