Figure 11: ROS generation was measured using the fluorescent dye 2,7 dichlorodihydrofluorescein diacetate after treatment with (a) different concentrations (12.5, 25 and 50 μg/mL) of synthesized Ag NPs for 24 h and its fluorescence was measured using a flow cytometer (b) Graph shows mean fluorescence intensity of DCF in control and synthesized Ag NPs treated cells at 24 h.