Figure 4: Non specific adsorption of BSA on a patterned gold surface in a fluidic cell. a) Schematic view of the setup. The fluidic cell is sealed by a glass window supporting identical biochip spots. In immobilization or affinity measurement experiment, the capture of the ligands attaching to the spots increases the optical thickness of the spot, which produces a change in the reflected light intensity. The method is widefield, all the spots are visualized at once. b) 3D reconstruction of an image obtained using the setup of Figure a. What represents this image is explained in the text. Numbers are arbitrary marks.