Figure 6: (A) siRNA knockdown of the eIF3b subunit induces cell cycle arrest in a similar manner to Ch-SeNPs in HeLa cells. The suitability of the transfection was validated by Western Blot using the methyl-tetrahydrofolate reductase (MTHFR1) as loading control since it showed a SILAC ratio closed to 1. (B) Silencing of the eiF3b expression partially increased the cell population in G2/M phase while decreased the population in the G0/G1 phase. The results obtained after knocking down eIF3b were similar to those obtained after Ch-SeNPs exposure. (C) The invasive behavior of HepG2 cells in a matrigel decrease in the presence of Ch-SeNPs. Cells exposed to 0.5 μg ml-1 Ch-SeNPs formed a less branched culture as compared to control cells. The morphology of cells exposed to 1.0 μg ml-1 Ch-SeNPs was very different from control cells, appearing round and individuals with no ramifications.