Figure 7: pDNA delivery in CHO cell line transfected with polymer/pDNA complexes at weight ratios of 5 &10 after 48 hrs incubation. [Z0 (10 µg), Z1-Z9 (5/10 µg), PEI 25k (5 µg); pDNA 1 µg in 500 µl medium in CHO cell line, (PEI 25k was tested with the dose of 5 µg, since 10 µg caused nearly 100% cell death). A) GFP expression illustrated under fluorescent microscopy. B) Transfection efficiency analyzed with FACS (*p<0.05 compared with PEI 25k, two-tailed Student’s t-test, error bar represents standard deviation, n=3). C) Cell viability (*p<0.05 compared with untreated cell as control, two-tailed Student’s t-test, error bar represents standard deviation, n=3).