Figure 5: Differential transcript activity in primary human liver stellate cells treated with ZnO nanoparticles or dissolved ZnSO4, at 30 μg/mL. Cells were treated in duplicate with ZnO nanoparticles or ZnSO4 for 24 h. A. Totals of differentially-expressed transcripts for each treatment grouped according to whether they were up-regulated (red) or down-regulated (green) compared to untreated cells. B. VENN diagrams showing whether differentially-expressed transcripts occurred uniquely within one treatment (unshared VENN), or were differentially activated by two or more of the treatments (intersecting VENN). For clarity, VENN treatments have been grouped into (i) uncoated ZnO nanoparticles or (ii) coated ZnO nanoparticles, both against an ionic zinc treatment (ZnSO4).