Plasma levels of dsDNA in mice treated with silica particles. BALB/c mice were injected intravenously with nSP70 (0.2, 0.4, or 0.8 mg/mouse), nSP300 (0.8 mg/mouse), mSP1000 (0.8 mg/mouse), or saline. BALB/c mice with no treatment were divided into untreated group. (A) The plasma concentrations of dsDNA were determined at 24 h after treatment. (B, C) The plasma concentration of dsDNA of each mouse was determined at 2 h (B) and 72 h (C) after treatment (0.8 mg/mouse). (D) BALB/c mice were injected intravenously with nSP70 (0.8 mg/mouse) or nSP70-N (0.8 mg/mouse), and the plasma concentration of dsDNA in each mouse was determined 24 h after treatment. (E) BALB/c mice were injected intraperitoneally with anti-Ly-6G antibodies (50, 100, or 150 µg/mouse), isotype controls (150 µg/mouse), or PBS 24 h prior to nSP70 injection (0.8 mg/mouse). The plasma concentration of dsDNA in each mouse was determined 24 h after nSP70 treatment. Data are presented as mean ± SEM; n = 5–6; **P < 0.01 vs saline-treated group; #P < 0.05 vs nSP70-treated group.