Variables Code Levels 1 2 Cross Linker X1 0.5 gm 1gm Drug X2 20 mg 50 mg Formula code X1 X2 Responses Particle diameter PDI Zeta Potential Entrapment efficiency (%)a F1 0.5 20 28.21 0.269 -12.2 66.07 F2 0.5 50 15.69 0.423 -35.9 74.24 F3 1 20 24.36 0.608 -10.6 80.48 F4 1 50 15.69 0.318 -7.67 91.31 aAverage of three experiments

Table 2: Experimental condition, design and responses of 22 factorial design preparation of ACV nanoparticles.