Figure 6 :Effect of lipid nanoparticle constituents and production parameters on the uptake by human brain endothelial hCMEC/D3 cells. Data represent the mean ± s.d. (n=3) of the concentration-dependent experiments at 37°C. Graphs show the dose response curve obtained treating the cells with the indicated dose of lipid nanoparticles constituted of solid lipids (A) or a mixture of solid and liquid lipids (B). Data refer to lipid nanoparticles previously incubated at 40°C, for 30 min, in the absence (open circles) or in the presence of polysorbate 80 (closed circles). For nanoparticle identification codes, composition and preparation procedure, please refer to Table 1 and experimental section.