Figure 8: UV-VIS absorption spectra of supernatant solutions of GNR-Ab (HER2) conjugates after incubation for six hours in heparinized mouse blood. Synthesis steps for protocols 1, 2 and 3 are the same as in figure 4. Before and after 1 h incubation all protocols for conjugated and pegylated (PEG and Prot. 1, Prot. 2 and Prot. 3, respectively) have similar spectra. After 6 h incubations a higher level of aggregation is observed in samples from Prot 3, with the best results for Prot.1 (lower aggregation)