Figure 4: Effect on gross tumor volume regression following intratumoral injection of the nanoparticles loaded with Bcl-2 siRNA, IGF-1R siRNA, p53 plasmid or IGF-1R/Bcl-2 siRNAs plus p53 plasmid. The mice bearing 4T1-induced mammary tumor were treated with 100 μl of the particle suspension originally formulated in 1 ml of DMEM with 7 mM of Ca2+ in presence or absence of 800 nm of each of the siRNAs plus 100 ng of p53, through intratumoral injection and the body weight was measured throughout the experiment from day 1 to day 29.