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Figure 3: The feasibility of KF polymerase-aided amplification assay. The fluorescence spectras of the proposed method were under different conditions. (a) Blank: buffer; (b) 50 nM HP + GO + SGI; (c) 50 nM HP + GO + SGI + KF; (d) 50 nM HP + HA + GO + SGI; (e) 50 nM HP + HA + GO + SGI + KF. The concentrations of HA protein and GO were respectively 5 μg/mL and 15 μg /mL.Excition: 496 nm, and emission: 520 nm. Error bars indicate standard deviation (n=3). |