Figure 6: Activation of caspases: In cells treated with 300 μM Rh2(H2cit)4-
loaded maghemite NPs [Magh-Rh2(H2cit)4´] and 0.019 mol/L (iron) maghemite
nanoparticles loaded with citrate (Magh-cit), active caspases 6 (shown in
green) and 7 (red fluorescence) were identified after 12 hours of treatment. By
overlapping brightfield and fluorescence images, the pyknotic nuclei is indicated
by an arrow, which is characteristic of apoptosis. In the control group, only the
nuclei of MCF-7 cells were labeled with DAPI (blue fluorescence).
