S.No. Batch code PLGA (mg) Acetone (ml) Lomustine (mg) Stabilizer solution(ml) 1 PD1 100mg PDLG5002 10 1 1:5, 1%w/v PVA 2 PD2 200mg PDLG5002 10 1 1:5, 1%w/v PVA 3 PD3 300mg PDLG5002 10 1 1:5, 1%w/v PVA 4 PD4 100mg PDLG7502 10 1 1:5, 1%w/v PVA 5 PD5 200mg PDLG7502 10 1 1:5, 1%w/v PVA 6 PD6 300mg PDLG7502 10 1 1:5, 1%w/v PVA 7 PD7 100mg PDLG5002 20 1 1:5, 1%w/v PVA 8 PD8 100mg PDLG5002 30 1 1:5, 1%w/v PVA 9 PD9 100mg PDLG5002 10 5 1:5, 1%w/v PVA 10 PD10 100mg PDLG5002 10 10 1:5, 1%w/v PVA 11 PD11 100mg PDLG5002 10 1 1:5, 3%w/v PVA 12 PD12 100mg PDLG5002 10 1 1:5, 1%w/v Pluronic F68 13 PD13 100mg PDLG5002 10 1 1:5, 3%w/v Pluronic F68 14 PD14 100mg PDLG5002 10 1 1:10, 1%w/v PVA 15 PD15 100mg PDLG5002 10 1 1:10,1%w/v Pluronic F68

L/G ratio of: For PDLG 5002 is 50:50; For PDLG 7502 is 75:25. Table 1: Components of various batches of fabricated lomustine nanoparticles.