S.No Batch code Particle size (nm) (Mean ± SD*) Polydispersity index (PDI) (Mean ± SD*) Zeta potential (mV) (Mean ± SD*) Drug content (mg) % EE (Mean ± SD*) 1 PD1 296.9 ± 12.2 0.135 ± 0.013 −0.964 ± 0.029 0.87 87% ± 0.76 2 PD2 316.2 ± 13.4 0.128 ± 0.009 −2.09 ± 0.063 0.92 92.4% ± 0.70 3, PD3 335.8 ± 10.6 0.122 ± 0.016 −8.14 ± 0.076 0.98 98.1% ± 0.67 4 PD4 318.9 ± 14.3 0.136 ± 0.012 −2.06 ± 0.012 0.88 88.3% ± 0.81 5 PD5 344.5 ± 13.6 0.127 ± 0.011 −1.29 ± 0.034 0.93 93% ± 0.66 6 PD6 370.3 ± 11.4 0.138 ± 0.006 −0.61 ± 0.012 0.98 98.6% ± 1.02 7 PD7 227.3 ± 13.5 0.131 ± 0.016 −0.471 ± 0.008 0.85 85% ± 0.86 8 PD8 198.5 ± 11.4 0.110 ± 0.014 −0.238 ± 0.017 0.80 80.3% ± 0.84 9 PD9 304.2 ± 12.5 0.117 ± 0.011 −7.78 ± 0.121 4.8 96% ± 0.82 10 PD10 307.8 ± 11.2 0.124 ± 0.008 −8.2 ± 0.018 9.95 99.5% ± 0.75 11 PD11 334.6 ± 10.7 0.156 ± 0.007 −4.44 ± 0.098 0.88 88% ± 0.63 12 PD12 177.2 ± 14.1 0.135 ± 0.018 −4.3 ± 0.26 0.81 81% ± 0.85 13 PD13 167.9 ± 11.5 0.143 ± 0.011 −5.1 ± 0.312 0.79 79% ± 0.68 14 PD14 298.4 ± 10.1 0.192 ± 0.007 −6.63 ± 0.148 0.86 88% ± 0.65 15 PD15 197.9 ± 13.2 0.278 ± 0.007 −5.8 ± 0.207 0.85 85% ± 0.9

n=3, SD* = Standard deviation. Table 2: Effect of various process parameters on particle size, size distribution, zeta potential, drug content and encapsulation efficiency.