Concentration (in pg/ml) of cytokines

Cytokine	Treatment	Concentration of cytokine (pg/ml) ± SEM	Fold change, with respect to
Cytokine	Treatment	Concentration of cytokine (pg/ml) ± SEM	Control	TG	TG-F
IL-2	Control	19.05 ± 0.15
	TG48	18.05 ± 0.15	(-) 1.06#
	TG48F	20.40 ± 0.70		(+) 1.13
	TG48MF	20.25 ± 1.15		(+) 1.12	(-) 1.01
IL-4	Control	15.25 ± 0.05
	TG48	15.65 ± 0.35	(+) 1.03
	TG48F	17.50 ± 0.20		(+) 1.12*
	TG48MF	19.85 ± 0.95		(+) 1.27*	(+) 1.13
IL-5	Control	14.60 ± 0.20
	TG48	13.85 ± 0.55	(-) 1.05
	TG48F	15.30 ± 0.30		(+) 1.10
	TG48MF	15.85 ± 1.75		(+) 1.14	(+) 1.04
IFN-γ	Control	10.75 ± 0.45
	TG48	12.10 ± 0.30	(+) 1.13
	TG48F	12.45 ± 1.65		(+) 1.03
	TG48MF	12.90 ± 1.60		(+) 1.07	(+) 1.04
TNF-α	Control	10.80 ± 1.10
	TG48	10.30 ± 0.30	(-) 1.05
	TG48F	10.55 ± 1.05		(+) 1.02
	TG48MF	10.60 ± 1.40		(+) 1.03	(+) 1.01

(#: p<0.05 versus Control; *: p<0.05 versus TG; *: p<0.05 versus TG-F)

Table 14: Concentration (in pg/ml) of cytokines IL-2, IL-4, IL-5, IFN-γ and TNF-α, in peripheral blood, after 48 h of treatment, assayed using BD CBA Mouse Th1/Th2 Cytokine Kit. Cytokine analysis shows an increase in the levels of the cytokines with both, fisetin and MCN+fisetin, compared to TG.