Figure 6: Pre-loaded TAMs transporting RVG-PTX-nanocomplexes to U87 cells.

RVG-PTX-nanocomplex pre-loaded TAMs (red, nano*-TAMs) were co-cultured with GFP-U87 (green). At first 12 h, fluorescence images showed that nano*- TAMs transported red RVG-PTX-nanocomplexes into U87 via cell-to-cell direct transportation (A, arrow). The red “nanocomplexes” co-localized with GFP resulted as yellow in U87 cell bodies (A and B). Enlarged image (C) showed the “red-nanocomplexes” transporting to green U87 by TAMs process connection.